RNA-seq of SY3h from Mungbean - SRA

SRX1891150: RNA-seq of SY3h from Mungbean
1 ILLUMINA (Illumina HiSeq 2500) run: 60.5M spots, 15.2G bases, 5Gb downloads

Design: Messenger RNAs (mRNAs) from the total RNA were isolated using Oligo (dT) and were randomly cleaved into short fragments. Then the first strand cDNAs were synthesized with random hexamer primers and followed by second strand cDNAs synthesis using DNA polymeraseⅠ(Takara, China) and RNase H (Takara, China). The double-stand cDNAs were purified and ligated to adaptors for paired-end sequencing. Three independent biological replicates for each sample were analyized. The quality and quantity of the libraries were detected using an Agilent 2100 Bioanalyzer and ABI real time RT-PCR. The qualified cDNA libraries were carried out by an Illumina HiSeq 2500 platform with PE125.

Submitted by: College of Biology and Environmental Sciences, Jishou University (College of Biology and Environmental Sciences, Jis)

Study: Vigna radiata cultivar:Zhonglu-1 Raw sequence reads

PRJNA327304 • SRP077637 • All experiments • All runs

show Abstracthide Abstract

As a legume sepecies, mungbean also has the charcateristic of desiccation tolerance. However, the molecular mechanism of mungbean seed desiccation tolerance is still largely unkown. In this study, we attempt to investigate the transcriptome dynamics of mungbean seed in response to dehydration using RNA-seq. Thus, understanding of mungbean extreme tolerance to desiccation and drought will aid the development of strategies for improving drought stress resistance in crops.

Sample: RNA-seq of SY3h from Mungbean

SAMN05330734 • SRS1534078 • All experiments • All runs

Organism: Vigna radiata

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RT-PCR

Layout: PAIRED

Spot descriptor:

1 forward126 reverse

Runs: 1 run, 60.5M spots, 15.2G bases, 5Gb

Run	# of Spots	# of Bases	Size	Published
SRR3735589	60,485,454	15.2G	5Gb	2016-12-01

ID:: 2737350

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